| 產品名稱 |
Formula / Liter |
Directions |
| m-Endo Agar (7724) |
Lactose.....................................................................9.4g
Tryptose.......................................................................5g
Enzymatic Digest of Casein......................................3.7g
Enzymatic Digest of Animal Tissue...........................3.7g
Sodium Sulfite...........................................................1.6g
Yeast Extract.............................................................1.2g
Potassium Phosphate, monobasic..............................1g
Basic Fuchsin............................................................0.8g
Sodium Deoxycholate...............................................0.1g
Sodium Lauryl Sulfate.............................................0.05g
Ager...........................................................................15g
Final pH: 7.2 ± 0.2 at 25°C |
1.Suspend 51 g of the medium in 1 liter of purified
water containing 20 mL of non-denatured
Ethanol.
2.Heat with frequent agitation and boil to
completely dissolve the medium.
3.Avoid overheating. DO NOT AUTOCLAVE. |
| m-Enterococcus Agar (7544) |
Enzymatic Digest of Casein.......................................10g
Enzymatic Digest of Soybean Meal..........................7.5g
Yeast Extract................................................................5g
Dextrose.......................................................................2g
Dipotassium Phosphate............................................0.2g
Sodium Azide............................................................0.4g
2, 3, 5-Triphenyl Tetrazolium Cloride.........................0.1g
Final pH: 7.2 ± 0.2 at 25°C |
1.Suspend 42 g of the medium in one
liter of purified water.
2.Heat with frequent agitation and boil for
one minute to completely dissolve the
medium.
3.DO NOT AUTOCLAVE. Cool to 45 -
50°C and dispense. |
| m-FC Agar (7397) |
Enzymatic Digest of Casein.......................................10g
Enzymatic Digest of Animal Tissue............................10g
Yeast Extract................................................................3g
Sodium Chloride..........................................................5g
Lactose...................................................................12.5g
Bile Salts...................................................................1.5g
Aniline Blue...............................................................0.1g
Agar...........................................................................15g
Final pH: 7.4 ± 0.2 at 25°C |
1.Suspend 52 g of the medium in 1 L of
purified water containing 10 mL of 1%
Rosolic Acid in 0.2N NaOH.
2.If necessary, adjust pH to 7.4 with 1N
HCI.
3.Heat with frequent agitation and boil for
one minute to completely dissolve the
medium.
4.Cool to 45 - 50°C and pour plates.
5.DO NOT AUTOCLAVE. |
| m-HPC Agar (7690) |
Peptone......................................................................20g
Gelatin........................................................................25g
Agar...........................................................................15g
Final pH: 7.1 ± 0.2 at 25°C
|
1.Suspend 60 g of the medium in one liter of
purified water.
2.Heat with frequent agitation and boil for one
minute to completely dissolve the medium.
3.Add 10 mL of Glycerol to 1 Liter of solution.
4.Autoclave at 121°C for 5 minutes. |
| m-TEC Agar (7421) |
Enzymatic Digest of Animal Tissue..............................5g
Yeast Extract................................................................3g
Lactose.......................................................................10g
Sodium Chloride........................................................7.5g
Potassium Phosphate...............................................4.3g
Sodium Lauryl Sulfate...............................................0.2g
Sodium Deoxycholate...............................................0.1g
Bromcresol Purple...................................................0.08g
Bromphenol Red.....................................................0.08g
Agar...........................................................................15g
Final pH: 7.3 ± 0.2 at 25°C |
1.Suspend 45.3 g of the medium in one liter of
purified water.
2.Heat with frequent agitation and boil to
completely dissolve the medium.
3.Autoclave at 121°C for 15 minutes.
4.Dispense 4 - 5 mL amounts into 10 x 50 mm
Petri dishes, allow to solidify.
|
| m-TGE Broth(7451) |
Enzymatic Digest of Casein.......................................10g
Beef Extract..................................................................6g
Dextrose.......................................................................2g
Final pH: 7.0 ± 0.2 at 25°C |
1.Dissolve 18 g of the medium in one liter of purified water.
2.Mix throughly.
3.Autoclave at 121°C for 15 minutes. |
| m-Green Yeast & Fungi Broth (7190) |
Enzymatic Digest of Casein.........................................5g
Enzymatic Digest of Animal Tissue..............................5g
Yeast Extract................................................................9g
Dextrose.....................................................................50g
Magnesium Sulfate....................................................2.1g
Potassium Phosphate...................................................2g
Diastase...................................................................0.05g
Thiamine..................................................................0.05g
Bromcresol Green..................................................0.026g
Final pH: 4.6 ± 0.2 at 25°C |
1.Dissolve 73 g of the medium in one liter of purified water.
2.Mix throughly.
3.Autoclave at 121°C for 10 minutes. |
